Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture.
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چکیده
منابع مشابه
Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture
A method is presented for isolating osteoblasts from newborn mouse calvaria without the use of digestive enzymes. The procedure is based on the ability of osteoblasts to migrate from bone onto small glass fragments (Jones, S.J., and A. Boyde, 1977, Cell Tissue Res., 184:179-193). The isolated cells were cultured for up to 14 d in Dulbecco's modified Eagle's medium supplemented with 10% fetal ca...
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X-linked hypophosphatemia (XLH) is caused by inactivating mutations of PEX, an endopeptidase of uncertain function. This defect is shared by Hyp mice, the murine homologue of the human disease, in which a 3' Pex deletion has been documented. In the present study, we report that immortalized osteoblasts derived from the simian virus 40 (SV40) transgenic Hyp mouse (TMOb- Hyp) have an impaired cap...
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Acollagenase inhibitor was identified in the culture medium of mouse calvaria after separation from mouse bone collagenase by Sephadex G-200 gel filtration equilibrated with 6M urea in Tris buffer. The simultaneous synthesis of both collagenase and inhibitor by mouse calvaria in tissue culture was consistent with the resuls of our previous study using achick bone culture system and otehr recent...
متن کاملComparison of Phenotypic Characterization between Differentiated Osteoblasts from Stem Cells and Calvaria Osteoblasts In vitro
BACKGROUND Characteristics of differentiated osteoblasts from adipose derived stem cells (ADSCs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. The aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro. METHODS In this study, mesenchymal stem c...
متن کاملcomparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro
background: characteristics of differentiated osteoblasts from adipose derived stem cells (adscs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. the aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro . methods: in this study, mesenchymal stem ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.96.3.639